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add ACSF page
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content/pages/acsf.md

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title: Patch Clamp External Solutions
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description: Common bath solutions used for whole-cell patch-clamp electrophysiology
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---
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### ACSF
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Standard ACSF used for brain slice experiments. Contains 133.8 mM chloride.
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Chemical | mM
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---|---
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NaCl | 126
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Dextrose | 11
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MgSO4 | 1.5
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KCl | 3
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NaH2PO4 | 1.2
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CaCl2 | 2.4
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NaHCO3 | 25
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### Dissecting Solution
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ACSF with less calcium and more magnesium to reduce spontaneous activity
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Chemical | mM
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---|---
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NaCl | 124
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Dextrose | 10
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MgSO4 | 3
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KCl | 2.5
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NaH2PO4 | 1.23
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CaCl2 | 1
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NaHCO3 | 25
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### Mg-Free ACSF
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Standard ACSF where MgSO4 is replaced by NaCl |
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Chemical | mM
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---|---
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NaCl | 127.5
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Dextrose | 11
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KCl | 3
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NaH2PO4 | 1.2
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CaCl2 | 2.4
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NaHCO3 | 25
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### Sucrose-Laden ACSF
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Dissecting solution where Na is replaced with sucrose to reduce uptake and lysis |
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Chemical | mM
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Sucrose | 205
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Dextrose | 10
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MgSO4 | 1
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KCl | 2
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NaH2PO4 | 1.25
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CaCl2 | 1
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NaHCO3 | 25

content/pages/internals.md

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title: Patch Clamp Internal Solutions
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description: Common internal solutions used for whole-cell patch-clamp electrophysiology
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description: Common pipette solutions used for whole-cell patch-clamp electrophysiology
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When a cell enters whole-cell patch-clamp configuration the pipette solution (also called internal solution) fills the the patched cell and largely replaces most of the cytoplasm. Physiological pipette solutions can be used seek to mimic the natural electrical properties of cells, or experiments can use non-physiological pipette solutions to block or enhance the current flow through specific channels.

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